CHEMICAL-STRUCTURE AND CONFORMATIONAL FEATURES OF CELL-WALL POLYSACCHARIDES ISOLATED FROM APHANOASCUS-MEPHITALUS AND RELATED SPECIES

被引：15

作者：

JIMENEZBARBERO, J ^{[1
]}

BERNABE, M ^{[1
]}

LEAL, JA ^{[1
]}

PRIETO, A ^{[1
]}

GOMEZMIRANDA, B ^{[1
]}

机构：

[1] CSIC, CTR INVEST BIOL, E-28006 MADRID, SPAIN

来源：

CARBOHYDRATE RESEARCH | 1993年 / 250卷 / 02期

关键词：

D O I：

10.1016/0008-6215(93)84007-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structures of cell-wall mannans isolated from Aphanoascus mephitalus, A. fulvescens, A. verrucosus, and A. reticulisporus have been investigated by chemical analyses and 1D and 2D H-1 and C-13 NMR techniques. It was found that all of them consists of a relatively simple comb-like structure of the disaccharide repeating block {--> 6)-[alpha-Man p-(1 --> 2)]-alpha-Man p-(1 -->}. The conformations around the alpha-(1 --> 2) and alpha-(1 --> 6) linkages in these kinds of polymers were also studied by using molecular mechanics and dynamics calculations, together with NOE data. The results are similar to those found within the oligosaccharide chains of glycoproteins, with a well-defined conformation for the alpha-(1 --> 2) linkage and a certain restriction around the alpha-(1 --> 6) bonding imposed by the 2-substitution.

引用

页码：289 / 299

页数：11

共 45 条

[11] REEVALUATION OF ROTAMER POPULATIONS FOR 1,6 LINKAGES - RECONCILIATION WITH POTENTIAL-ENERGY CALCULATIONS [J].