COMBINATORIAL RNA SPLICING ALTERS THE SURFACE-CHARGE ON THE NMDA RECEPTOR

被引：111

作者：

ANANTHARAM, V

PANCHAL, RG

WILSON, A

KOLCHINE, VV

TREISTMAN, SN

BAYLEY, H

机构：

[1] WORCESTER FDN EXPTL BIOL INC, 222 MAPLE AVE, SHREWSBURY, MA 01545 USA

[2] UNIV MASSACHUSETTS, MED CTR, DEPT PHARMACOL, WORCESTER, MA 01655 USA

来源：

FEBS LETTERS | 1992年 / 305卷 / 01期

关键词：

RECEPTOR; CHANNEL; EXCITATORY AMINO ACID; GLUTAMATE; RNA SPLICING;

D O I：

10.1016/0014-5793(92)80648-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcripts encoding four NMDA receptor subunits, generated from the NMDAR1 gene by alternative RNA splicing, have been demonstrated in adult rat brain. RNA transcripts derived from cDNAs encoding each form direct the formation of functional NMDA receptors in Xenopus oocytes. The two amino acid cassettes of 21 and 37 amino acids found in the splice variants increase the positive extracellular surface charge on the subunits and may thereby modulate the functional properties of the receptor.

引用

页码：27 / 30

页数：4

共 21 条

[1] ABSOLUTE MESSENGER-RNA QUANTIFICATION USING THE POLYMERASE CHAIN-REACTION (PCR) - A NOVEL-APPROACH BY A PCR AIDED TRANSCRIPT TITRATION ASSAY (PATTY) [J].