ICSB - A SHIGELLA-FLEXNERI VIRULENCE GENE NECESSARY FOR THE LYSIS OF PROTRUSIONS DURING INTERCELLULAR SPREAD

被引：155
作者：
ALLAOUI, A
MOUNIER, J
PREVOST, MC
SANSONETTI, PJ
PARSOT, C
机构：
[1] INST PASTEUR, UNITE PATHOGENIE MICROBIENNE MOLEC,INSERM, UNITE 199,28 RUE DR ROUX, F-75724 PARIS 15, FRANCE
[2] INST PASTEUR, STN CENT MICROSCOPIE ELECTR, F-75724 PARIS 15, FRANCE
来源：
MOLECULAR MICROBIOLOGY | 1992年 / 6卷 / 12期
关键词：
D O I：
10.1111/j.1365-2958.1992.tb00885.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. We have characterized the icsB gene which is located on the virulence plasmid pWR100. After inactivation of icsB, the mutant strain remained invasive, but formed abnormally small plaques on HeLa cell monolayers, colonized only the peripheral cells of Caco-2 islets, and was unable to provoke a keratoconjunctivitis in guinea-pigs. Examination of infected HeLa cells showed that the icsB mutant was able to lyse the phagocytic vacuole and to form protrusions at the surface of infected cells, but, unlike the wild type, remained trapped in protrusions surrounded by two membranes. These results indicate that icsB is involved in the lysis of the protrusions, a step necessary for intercellular spread.
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页码：1605 / 1616
页数：12
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