HIGH-PERFORMANCE SEPARATIONS OF NUCLEIC-ACIDS USING CAPILLARY ELECTROPHORESIS AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：0
作者：
BABA, Y
TSUHAKO, M
ENOMOTO, S
CHIN, AM
DUBROW, RS
机构：
[1] APPL BIOSYST JAPAN INC,KOTO KU,TOKYO 136,JAPAN
[2] APPL BIOSYST INC,SAN JOSE,CA 95134
来源：
HRC-JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY | 1991年 / 14卷 / 03期
关键词：
CAPILLARY ELECTROPHORESIS; OLIGONUCLEOTIDES; NUCLEIC ACIDS; COMPARISON OF HPCE AND HPLC;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
High resolution separations of nucleic acids have been performed using high performance capillary electrophoresis (HPCE) and high performance liquid chromatography (HPLC). Electropherograms showing HPCE separations of single and double stranded DNA are presented and compared with HPLC separations. Single base resolution of poly(dA) oligonucleotides in the size range of 12 to 60-mers was achieved in 35 min using HPCE. Plate numbers for HPCE are in the hundreds of thousands and reproducibility is about 1-2% (RSD). In comparison with HPLC separations, the resolution of nucleic acids obtained using HPCE is much better than that using HPLC, while reproducibility of HPCE is comparable with that of HPLC.
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页码：204 / 206
页数：3
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