METABOLISM OF BILE-ACIDS .8. RAPID AND SENSITIVE METHOD FOR THE DETERMINATION OF THE LITHOGENIC INDEX OF BILE BY THIN-LAYER CHROMATOGRAPHY AND DENSITOMETRY USING A DUAL-WAVELENGTH CHROMATOSCANNER

被引：12

作者：

KIBE, A

KURAMOTO, T

HOSHITA, T

机构：

[1] Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine, Hiroshima, 734

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 100卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90124-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive method for quantitative determination of three bile lipid components, cholesterol, bile acids, and lecithin, is described. These components were separated by thin-layer chromatography on a silica gel plate, spots were visualized with a phosphomolybdate reagent, and their color intensities were estimated by direct densitometry using a dualwavelength chromatoscanner. The lithogenic index was estimated by the molar ratio of the three lipids. This method can be applied to the routine analysis of bile in patients with gallstones. It has been evaluated by comparison with the method using standard gas-liquid chromatography and spectrophotometry. © 1979.

引用

页码：146 / 151

页数：6

共 17 条

[11] CHOLESTEROL SOLUBILITY IN BILE - EVIDENCE THAT SUPERSATURATED BILE IS FREQUENT IN HEALTHY MAN [J].