CLONING OF A MOUSE CDNA-ENCODING DNA-POLYMERASE-DELTA - REFINEMENT OF THE HOMOLOGY BOXES

被引：32

作者：

CULLMANN, G ^{[1
]}

HINDGES, R ^{[1
]}

BERCHTOLD, MW ^{[1
]}

HUBSCHER, U ^{[1
]}

机构：

[1] UNIV ZURICH IRCHEL,INST VET BIOCHEM,CH-8057 ZURICH,SWITZERLAND

来源：

GENE | 1993年 / 134卷 / 02期

关键词：

ANTIBODIES; DNA REPLICATION; PCR; SEQUENCE COMPARISON; EVOLUTIONARY CONSERVATION;

D O I：

10.1016/0378-1119(93)90093-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A mouse DNA polymerase delta (Pol delta)-encoding cDNA (pol delta) was isolated by PCR amplification and cDNA library screening. The sequenced cDNA has a length of 3386 bp and the open reading frame (ORF) encodes a protein of 1105 amino acids (aa) with an M(r) of 123 743. The aa identity to the proteins encoded by the corresponding cDNA from Bos taurus (93%) and Homo sapiens (92%) is very high. The identity to the Pol delta from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Plasmodium falciparum is around 50%. An aa comparison between all available Pol delta sequences reveals several common structural motifs. Polyclonal antibodies raised against a 31-aa synthetic peptide deduced from the ORF specifically recognize Pol delta polymerases from human cells and calf thymus in an immunoblot.

引用

页码：191 / 200

页数：10

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