SEPARATION OF CHLOROPHYLLS-C1, CHLOROPHYLLS-C2, AND CHLOROPHYLLS-C3 OF MARINE-PHYTOPLANKTON BY REVERSED-PHASE-C18 HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：217

作者：

KRAAY, GW ^{[1
]}

ZAPATA, M ^{[1
]}

VELDHUIS, MJW ^{[1
]}

机构：

[1] CTR EXPTL VILAXOAN CONSELLERIA PESCA,E-36600 VILAGARCIA AROUSA,SPAIN

来源：

JOURNAL OF PHYCOLOGY | 1992年 / 28卷 / 05期

关键词：

CHLOROPHYLLS-C1; CHLOROPHYLLS-C2; AND CHLOROPHYLLS-C3; CHROMOPHYTE PHYTOPLANKTON; PIGMENT ANALYSIS; RP-C18-HPLC;

D O I：

10.1111/j.0022-3646.1992.00708.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We separated chlorophylls c1, c2, and c3 of marine phytoplankton together with other pigments by a modification of the commonly applied reversed-phase-C18-high-performance liquid chromatography (RP-C18-HPLC) method. However, the chlorophyll c-like pigment 2,4, Mg-divinylpheoporphyrin a5 monomethyl ester, co-eluted with chlorophyll c1. The method involves optimization of the mobile phase by using a very high ion strength solvent in combination with a high carbon loaded RP-C18 column. Fingerprints of the various taxonomic groups of algae can thus be developed in a single run, including separation of the carotenoids lutein and zeaxanthin.

引用

页码：708 / 712

页数：5

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