PARAMETRIC OPTIMIZATION OF EXTRACELLULAR ALPHA-AMYLASE PRODUCTION BY THERMOPHILIC BACILLUS-COAGULANS

被引：34
|
作者：
BABU, KR
SATYANARAYANA, T
机构：
[1] Department of Microbiology, University of Delhi South Campus, New Delhi
来源：
FOLIA MICROBIOLOGICA | 1993年 / 38卷 / 01期
关键词：
D O I：
10.1007/BF02814555
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The culture parameters required for optimum production of extracellular alpha-amylase by the thermophilic Bacillus coagulans are described. The optimum pH, temperature and incubation period for amylase production were 7, 50-degrees-C and 48 h, respectively. Age of inoculum (48 h) and its level (2 %) were critical for maximum amylase yield. The enzyme secretion was high in rice starch and beef extract as compared to other carbon and nitrogen sources tested. The addition of mustard oil cake (1 %) and agitation at 1.7 Hz resulted in an enhancement of alpha-amylase secretion.
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页码：77 / 80
页数：4
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