CELL CYCLE-DEPENDENT CHANGES IN TISSUE TRANSGLUTAMINASE MESSENGER-RNA LEVELS IN BOVINE ENDOTHELIAL-CELLS

被引：8

作者：

NARA, K

AOYAMA, Y

IWATA, T

HAGIWARA, H

HIROSE, S

机构：

[1] Department of Biological Sciences, Tokyo Institute of Technology, Meguroku, Tokyo, 152, Ookayama

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 187卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)81451-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The pattern of transglutaminase gene expression through the cell cycle was examined by Northern blot analysis using cultured bovine endothelial cells and a cDNA probe. When the cells reached confluency or were arrested in G0/G1 phase by nutrition deprivation, transglutaminase mRNA rose to a very high level; S- and M-phase extracts showed high and low levels, respectively. Subcellular localization studies by sucrose gradient centrifugation and immunostaining demonstrated that the majority of transglutaminase is present in cytosols throughout the cycle. The cell cycle-dependent changes in the transglutaminase mRNA levels strongly support the implicated involvement of the enzyme in cell growth, differentiation, and senescence. © 1992 Academic Press, Inc.

引用

页码：14 / 17

页数：4

共 20 条

[1] ALTERATIONS IN THE DISTRIBUTION AND ACTIVITY OF TRANSGLUTAMINASE DURING TUMOR-GROWTH AND METASTASIS [J].

BARNES, RN ;

BUNGAY, PJ ;

ELLIOTT, BM ;

WALTON, PL ;

GRIFFIN, M .

CARCINOGENESIS, 1985, 6 (03) :459-463

[2] NUCLEI FROM RAT LIVER - ISOLATION METHOD THAT COMBINES PURITY WITH HIGH YIELD [J].

BLOBEL, G ;

POTTER, VR .

SCIENCE, 1966, 154 (3757) :1662-&

[3] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[4] RAPID INCREASES IN THE TRANSGLUTAMINASE ACTIVITY OF A431 CELLS FOLLOWING TREATMENT WITH EPIDERMAL GROWTH-FACTOR [J].

DADABAY, CY ;

PIKE, LJ .

BIOCHEMISTRY, 1987, 26 (21) :6587-6591

[5]

DAVIES PJA, 1985, J BIOL CHEM, V260, P5166

[6] TRANSGLUTAMINASES [J].

FOLK, JE .

ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 :517-531

[7] TRANSGLUTAMINASES - MULTIFUNCTIONAL CROSS-LINKING ENZYMES THAT STABILIZE TISSUES [J].

GREENBERG, CS ;

BIRCKBICHLER, PJ ;

RICE, RH .

FASEB JOURNAL, 1991, 5 (15) :3071-3077

[8] SITOSTEROL-STIMULATIVE PRODUCTION OF PLASMINOGEN-ACTIVATOR IN CULTURED ENDOTHELIAL-CELLS FROM BOVINE CAROTID-ARTERY [J].

HAGIWARA, H ;

SHIMONAKA, M ;

MORISAKI, M ;

IKEKAWA, N ;

INADA, Y .

THROMBOSIS RESEARCH, 1984, 33 (04) :363-370

[9] CHANGES IN TRANSGLUTAMINASE ACTIVITY IN CARBON TETRACHLORIDE-DAMAGED RAT-LIVER [J].

KOHNO, H ;

KASHIMURA, K ;

KATOH, S ;

OHKUBO, Y .

EXPERIENTIA, 1991, 47 (01) :70-75

[10] BOVINE AORTIC ENDOTHELIAL-CELL TRANSGLUTAMINASE - ENZYME CHARACTERIZATION AND REGULATION OF ACTIVITY [J].

KORNER, G ;

SCHNEIDER, DE ;

PURDON, MA ;

BJORNSSON, TD .

BIOCHEMICAL JOURNAL, 1989, 262 (02) :633-641

← 1 2 →