CLONING AND EXPRESSION IN YEAST OF A PLANT POTASSIUM-ION TRANSPORT-SYSTEM

被引：569
作者：
SENTENAC, H
BONNEAUD, N
MINET, M
LACROUTE, F
SALMON, JM
GAYMARD, F
GRIGNON, C
机构：
[1] CNRS,CTR GENET MOLEC,F-91198 GIF SUR YVETTE,FRANCE
[2] INRA,MICROBIOL IPV,F-34060 MONTPELLIER 1,FRANCE
来源：
SCIENCE | 1992年 / 256卷 / 5057期
关键词：
D O I：
10.1126/science.1585180
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or Rb-86(+)) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
引用
收藏
页码：663 / 665
页数：3
相关论文
共 32 条
  • [1] MOLECULAR-CLONING AND NUCLEOTIDE SEQUENCING OF THE GENE FOR ESCHERICHIA-COLI CAMP RECEPTOR PROTEIN
    AIBA, H
    FUJIMOTO, S
    OZAKI, N
    [J]. NUCLEIC ACIDS RESEARCH, 1982, 10 (04) : 1345 - 1361
  • [2] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF A CYCLIC NUCLEOTIDE-ACTIVATED CHANNEL FROM OLFACTORY NEURONS
    DHALLAN, RS
    YAU, KW
    SCHRADER, KA
    REED, RR
    [J]. NATURE, 1990, 347 (6289) : 184 - 187
  • [3] TRK1 ENCODES A PLASMA-MEMBRANE PROTEIN REQUIRED FOR HIGH-AFFINITY POTASSIUM-TRANSPORT IN SACCHAROMYCES-CEREVISIAE
    GABER, RF
    STYLES, CA
    FINK, GR
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (07) : 2848 - 2859
  • [4] CHARACTERIZATION OF AN IMMEDIATE-EARLY GENE INDUCED IN ADHERENT MONOCYTES THAT ENCODES I-KAPPA-B-LIKE ACTIVITY
    HASKILL, S
    BEG, AA
    TOMPKINS, SM
    MORRIS, JS
    YUROCHKO, AD
    SAMPSONJOHANNES, A
    MONDAL, K
    RALPH, P
    BALDWIN, AS
    [J]. CELL, 1991, 65 (07) : 1281 - 1289
  • [5] THE PHYSIOLOGY OF ION CHANNELS AND ELECTROGENIC PUMPS IN HIGHER-PLANTS
    HEDRICH, R
    SCHROEDER, JI
    [J]. ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1989, 40 : 539 - 569
  • [6] SEQUENCE HOMOLOGY BETWEEN 2 MEMBRANE-TRANSPORT ATPASES, THE KDP-ATPASE OF ESCHERICHIA-COLI AND THE CA-2+-ATPASE OF SARCOPLASMIC-RETICULUM
    HESSE, JE
    WIECZOREK, L
    ALTENDORF, K
    REICIN, AS
    DORUS, E
    EPSTEIN, W
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (15): : 4746 - 4750
  • [7] VOLTAGE-SENSITIVE ION CHANNELS
    JAN, LY
    JAN, YN
    [J]. CELL, 1989, 56 (01) : 13 - 25
  • [8] JAN LY, 1990, NATURE, V345, P673
  • [9] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION FROM COMPLEMENTARY-DNA OF THE ROD PHOTORECEPTOR CYCLIC GMP-GATED CHANNEL
    KAUPP, UB
    NIIDOME, T
    TANABE, T
    TERADA, S
    BONIGK, W
    STUHMER, W
    COOK, NJ
    KANGAWA, K
    MATSUO, H
    HIROSE, T
    MIYATA, T
    NUMA, S
    [J]. NATURE, 1989, 342 (6251) : 762 - 766
  • [10] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN
    KYTE, J
    DOOLITTLE, RF
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) : 105 - 132
1234