CLONING AND EXPRESSION IN YEAST OF A PLANT POTASSIUM-ION TRANSPORT-SYSTEM

被引：573
作者：
SENTENAC, H
BONNEAUD, N
MINET, M
LACROUTE, F
SALMON, JM
GAYMARD, F
GRIGNON, C
机构：
[1] CNRS,CTR GENET MOLEC,F-91198 GIF SUR YVETTE,FRANCE
[2] INRA,MICROBIOL IPV,F-34060 MONTPELLIER 1,FRANCE
来源：
SCIENCE | 1992年 / 256卷 / 5057期
关键词：
D O I：
10.1126/science.1585180
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micromolar range and to absorb K+ (or Rb-86(+)) at rates similar to those in wild-type yeast. The predicted amino acid sequence (838 amino acids) has three domains: a channel-forming region homologous to animal K+ channels, a cyclic nucleotide-binding site, and an ankyrin-like region.
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页码：663 / 665
页数：3
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