CLONING OF A HUMAN GENE ENCODING THE GENERAL TRANSCRIPTION INITIATION FACTOR-IIB

被引：245
作者：
HA, I
LANE, WS
REINBERG, D
机构：
[1] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT BIOCHEM,675 HOES LANE,PISCATAWAY,NJ 08854
[2] HARVARD UNIV,HARVARD MICROCHEM FACIL,CAMBRIDGE,MA 02138
来源：
NATURE | 1991年 / 352卷 / 6337期
关键词：
D O I：
10.1038/352689a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.
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页码：689 / 695
页数：7
相关论文
共 51 条
  • [41] DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS
    SOUTHERN, EM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) : 503 - +
  • [42] STONE KL, 1989, TECHNIQUES PROTEIN C, P377
  • [43] DIRECT AND SELECTIVE BINDING OF AN ACIDIC TRANSCRIPTIONAL ACTIVATION DOMAIN TO THE TATA-BOX FACTOR TFIID
    STRINGER, KF
    INGLES, CJ
    GREENBLATT, J
    [J]. NATURE, 1990, 345 (6278) : 783 - 786
  • [44] STUKA R, 1990, FEBS LETT, V261, P223
  • [45] FACTORS INVOLVED IN SPECIFIC TRANSCRIPTION BY MAMMALIAN RNA POLYMERASE-II - IDENTIFICATION OF GENERAL TRANSCRIPTION FACTOR TFIIG
    SUMIMOTO, H
    OHKUMA, Y
    YAMAMOTO, T
    HORIKOSHI, M
    ROEDER, RG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (23) : 9158 - 9162
  • [46] COMPLETION OF THE RABIES VIRUS GENOME SEQUENCE DETERMINATION - HIGHLY CONSERVED DOMAINS AMONG THE L (POLYMERASE) PROTEINS OF UNSEGMENTED NEGATIVE-STRAND RNA VIRUSES
    TORDO, N
    POCH, O
    ERMINE, A
    KEITH, G
    ROUGEON, F
    [J]. VIROLOGY, 1988, 165 (02) : 565 - 576
  • [47] WEIL PA, 1979, CELL, V18, P469
  • [48] COMPARISON OF THE 3 LARGE POLYMERASE PROTEINS OF INFLUENZA A, B, AND C VIRUSES
    YAMASHITA, M
    KRYSTAL, M
    PALESE, P
    [J]. VIROLOGY, 1989, 171 (02) : 458 - 466
  • [49] NUCLEOTIDE-SEQUENCE ANALYSIS OF THE L-GENE OF NEWCASTLE-DISEASE VIRUS - HOMOLOGIES WITH SENDAI AND VESICULAR STOMATITIS VIRUSES
    YUSOFF, K
    MILLAR, NS
    CHAMBERS, P
    EMMERSON, PT
    [J]. NUCLEIC ACIDS RESEARCH, 1987, 15 (10) : 3961 - 3976
  • [50] SEQUENCING AND EXPRESSION OF COMPLEMENTARY-DNA FOR THE GENERAL TRANSCRIPTION FACTOR-BTF3
    ZHENG, XM
    BLACK, D
    CHAMBON, P
    EGLY, JM
    [J]. NATURE, 1990, 344 (6266) : 556 - 559
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