CLONING OF A HUMAN GENE ENCODING THE GENERAL TRANSCRIPTION INITIATION FACTOR-IIB

被引：245
作者：
HA, I
LANE, WS
REINBERG, D
机构：
[1] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT BIOCHEM,675 HOES LANE,PISCATAWAY,NJ 08854
[2] HARVARD UNIV,HARVARD MICROCHEM FACIL,CAMBRIDGE,MA 02138
来源：
NATURE | 1991年 / 352卷 / 6337期
关键词：
D O I：
10.1038/352689a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.
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页码：689 / 695
页数：7
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