ABSOLUTE QUANTIFICATION OF INTRACELLULAR NA+ USING TRIPLE-QUANTUM-FILTERED NA-23 NMR

被引：45

作者：

ALLIS, JL

SEYMOUR, AML

RADDA, GK

机构：

[1] JOHN RADCLIFFE HOSP,MRC,BIOCHEM & CLIN MAGNET RESONANCE UNIT,OXFORD OX3 9DU,ENGLAND

[2] UNIV OXFORD,DEPT BIOCHEM,OXFORD,ENGLAND

来源：

JOURNAL OF MAGNETIC RESONANCE | 1991年 / 93卷 / 01期

关键词：

D O I：

10.1016/0022-2364(91)90032-O

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a method for the absolute quantification of triple-quantum-filtered spectra of spin-3/2 nuclei. The method is illustrated by measuring the amount of intracellular Na+ in the perfused rat heart. There is good agreement between the result determined by the triple-quantum method and that measured using a simple single-quantum pulse-and-collect experiment. © 1991.

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页码：71 / 76

页数：6

相关论文

共 18 条

[1] A STUDY OF TRANSVERSE RELAXATION OF RB-87 IN AGAROSE GELS BY TRIPLE-QUANTUM FILTRATION [J].

ALLIS, JL ;

DIXON, RM ;

RADDA, GK .

JOURNAL OF MAGNETIC RESONANCE, 1990, 90 (01) :141-147

[2]

ALLIS JL, 1989, THESIS OXFORD U

[3] SUPPRESSION OF ARTIFACTS IN 2-DIMENSIONAL J SPECTROSCOPY [J].

BODENHAUSEN, G ;

FREEMAN, R ;

TURNER, DL .

JOURNAL OF MAGNETIC RESONANCE, 1977, 27 (03) :511-514

[4] ANALYSIS OF NETWORKS OF COUPLED SPINS BY MULTIPLE QUANTUM NMR [J].

BRAUNSCHWEILER, L ;

BODENHAUSEN, G ;

ERNST, RR .

MOLECULAR PHYSICS, 1983, 48 (03) :535-560

[5] AQUEOUS SHIFT-REAGENTS FOR HIGH-RESOLUTION CATIONIC NUCLEAR MAGNETIC-RESONANCE .3. DY(TTHA)3-, TM(TTHA)3-, AND TM(PPP)27- [J].

CHU, SC ;

PIKE, MM ;

FOSSEL, ET ;

SMITH, TW ;

BALSCHI, JA ;

SPRINGER, CS .

JOURNAL OF MAGNETIC RESONANCE, 1984, 56 (01) :33-47

[6] TOXICITY OF DYSPROSIUM SHIFT-REAGENTS IN THE ISOLATED PERFUSED RAT-KIDNEY [J].

ENDRE, ZH ;

ALLIS, JL ;

RADDA, GK .

MAGNETIC RESONANCE IN MEDICINE, 1989, 11 (02) :267-274

[7] OBSERVATION OF INTRACELLULAR POTASSIUM AND SODIUM IN THE HEART BY NMR - A MAJOR FRACTION OF POTASSIUM IS INVISIBLE [J].

FOSSEL, ET ;

HOEFELER, H .

MAGNETIC RESONANCE IN MEDICINE, 1986, 3 (04) :534-540

[8] DIRECT OBSERVATION OF RESOLVED RESONANCES FROM INTRA-CELLULAR AND EXTRACELLULAR NA-23 IONS IN NMR-STUDIES OF INTACT-CELLS AND TISSUES USING DYSPROSIUM(III)TRIPOLYPHOSPHATE AS PARAMAGNETIC SHIFT-REAGENT [J].

GUPTA, RK ;

GUPTA, P .

JOURNAL OF MAGNETIC RESONANCE, 1982, 47 (02) :344-350

[9] NONEXPONENTIAL NUCLEAR MAGNETIC RELAXATION BY QUADRUPOLE INTERACTIONS [J].

HUBBARD, PS .

JOURNAL OF CHEMICAL PHYSICS, 1970, 53 (03) :985-&

[10] MULTIPLE-QUANTUM NMR-SPECTROSCOPY OF S=3/2 SPINS IN ISOTROPIC-PHASE - A NEW PROBE FOR MULTIEXPONENTIAL RELAXATION [J].

JACCARD, G ;

WIMPERIS, S ;

BODENHAUSEN, G .

JOURNAL OF CHEMICAL PHYSICS, 1986, 85 (11) :6282-6293