IMMOBILIZATION OF PREGASTRIC ESTERASES IN A HOLLOW-FIBER REACTOR FOR CONTINUOUS PRODUCTION OF LIPOLYSED BUTTEROIL

被引：15

作者：

GARCIA, HS ^{[1
]}

QURESHI, A ^{[1
]}

LESSARD, L ^{[1
]}

GHANNOUCHI, S ^{[1
]}

HILL, CG ^{[1
]}

机构：

[1] UNIV WISCONSIN,DEPT CHEM ENGN,MADISON,WI 53706

来源：

FOOD SCIENCE AND TECHNOLOGY-LEBENSMITTEL-WISSENSCHAFT & TECHNOLOGIE | 1995年 / 28卷 / 03期

关键词：

D O I：

10.1016/S0023-6438(95)94035-9

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Lingual lipases from calf; lamb and goat were semi-purified by cold storage and microtitration, and subsequently immobilized by physical adsorption on hollow fibers fabricated of microporous polypropylene. The immobilized lipases were employed in a hollow fiber membrane reactor to effect the continuous hydrolysis of a butterfat fraction that remains liquid at 18 degrees C. The goat lipase showed higher lipolytic activity than the other two but all three enzymes had good selectivities for short-chain fatty acids. No deactivation was observed for any of the immobilized enzymes during 5 d of continuous operation. A process based on this technology offers a number of advantages for the commercial production of lipolysed butteroil.

引用

页码：253 / 258

页数：6

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