CAPILLARY ELECTROPHORESIS ELECTROSPRAY-IONIZATION ION-TRAP MASS-SPECTROMETRY USING A SHEATHLESS INTERFACE

被引:39
作者
RAMSEY, RS
MCLUCKEY, SA
机构
[1] Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee
关键词
MASS SPECTROMETRY; ONLINE CAPILLARY ELECTROPHORESIS; MS/MS ANALYSIS; SHEATHLESS ELECTROSPRAY SOURCE; PEPTIDES;
D O I
10.1002/mcs.1220070505
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The sensitivity that can be obtained with the quadrupole ion trap for on-line CE analysis using a sheathless electrospray interface and gold-tipped columns is examined. Capillaries of different i.d. are compared in continuous infusion experiments and the effects of sheath solvents on the signal for some peptides and proteins are illustrated. The sheathless CE/ITMS system using 20 mu m i.d. columns is shown to provide mid-attomole detection levels for leucine enkephalin in total ion electropherograms under full scan conditions. Good quality MS/MS spectra are also obtained for low femtomole injections of this peptide. (C) 1995 John Wiley & Sons, Inc.
引用
收藏
页码:461 / 469
页数:9
相关论文
共 50 条
[41]   Design of a sheathless capillary electrophoresis-mass spectrometry probe for operation with a Z-Spray™ ionization source [J].
McComb, ME ;
Perreault, H .
ELECTROPHORESIS, 2000, 21 (07) :1354-1362
[42]   On-line sheathless capillary electrophoresis/nanoelectrospray ionization-tandem mass spectrometry for the analysis of glycosaminoglycan oligosaccharides [J].
Zamfir, A ;
Seidler, DG ;
Schönherr, E ;
Kresse, H ;
Peter-Katalinic, J .
ELECTROPHORESIS, 2004, 25 (13) :2010-2016
[43]   DEUTERIUM-EXCHANGE OF ALPHA-HELICES AND BETA-SHEETS AS MONITORED BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY [J].
WAGNER, DS ;
MELTON, LG ;
YAN, YB ;
ERICKSON, BW ;
ANDEREGG, RJ .
PROTEIN SCIENCE, 1994, 3 (08) :1305-1314
[44]   Determination of yessotoxins and pectenotoxins in shellfish by capillary electrophoresis-electrospray ionization-mass spectrometry [J].
de la Iglesia, P. ;
Gago-Martinez, A. .
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2009, 26 (02) :221-228
[45]   Straight nano-electrospray ionization and its coupling of mobility capillary electrophoresis to mass spectrometry [J].
Zhang, Fei ;
Hong, Jie ;
Xu, Wei ;
Qu, Feng .
TALANTA, 2020, 206
[46]   REDUCED ELUTION SPEED DETECTION FOR CAPILLARY ELECTROPHORESIS MASS-SPECTROMETRY [J].
GOODLETT, DR ;
WAHL, JH ;
UDSETH, HR ;
SMITH, RD .
JOURNAL OF MICROCOLUMN SEPARATIONS, 1993, 5 (01) :57-62
[47]   Feasibility of nonvolatile buffers in capillary electrophoresis-electrospray ionization-mass spectrometry of proteins [J].
Eriksson, JHC ;
Mol, R ;
Somsen, GW ;
Hinrichs, WLJ ;
Frijlink, HW ;
de Jong, GJ .
ELECTROPHORESIS, 2004, 25 (01) :43-49
[48]   Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry [J].
Nemes, Peter ;
Rubakhin, Stanislav S. ;
Aerts, Jordan T. ;
Sweedler, Jonathan V. .
NATURE PROTOCOLS, 2013, 8 (04) :783-799
[49]   Analysis of low-molecular-mass organic acids using capillary zone electrophoresis-electrospray ionization mass spectrometry [J].
Hagberg, J .
JOURNAL OF CHROMATOGRAPHY A, 2003, 988 (01) :127-133
[50]   Electrospray interface for capillary electrophoresis mass spectrometry with fiber-optic UV detection close to the electrospray tip [J].
Foret, F ;
Kirby, DP ;
Vouros, P ;
Karger, BL .
ELECTROPHORESIS, 1996, 17 (12) :1829-1832