CAPILLARY ELECTROPHORESIS ELECTROSPRAY-IONIZATION ION-TRAP MASS-SPECTROMETRY USING A SHEATHLESS INTERFACE

被引：39
作者：
RAMSEY, RS
MCLUCKEY, SA
机构：
[1] Chemical and Analytical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee
来源：
JOURNAL OF MICROCOLUMN SEPARATIONS | 1995年 / 7卷 / 05期
关键词：
MASS SPECTROMETRY; ONLINE CAPILLARY ELECTROPHORESIS; MS/MS ANALYSIS; SHEATHLESS ELECTROSPRAY SOURCE; PEPTIDES;
D O I：
10.1002/mcs.1220070505
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The sensitivity that can be obtained with the quadrupole ion trap for on-line CE analysis using a sheathless electrospray interface and gold-tipped columns is examined. Capillaries of different i.d. are compared in continuous infusion experiments and the effects of sheath solvents on the signal for some peptides and proteins are illustrated. The sheathless CE/ITMS system using 20 mu m i.d. columns is shown to provide mid-attomole detection levels for leucine enkephalin in total ion electropherograms under full scan conditions. Good quality MS/MS spectra are also obtained for low femtomole injections of this peptide. (C) 1995 John Wiley & Sons, Inc.
引用
收藏
页码：461 / 469
页数：9
相关论文
共 50 条
[31]   A novel two-laser interface for coupling capillary electrochromatography with ion-trap time-of-flight mass spectrometry [J].
Simpson, David C. ;
Yates, Alexander J. ;
Knox, John H. ;
Langridge-Smith, Pat R. R. .
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2014, 363 :8-15
[32]   Capillary electrophoresis electrospray ionization-mass spectrometry for peptidomics-based processing site determination [J].
Tsuchiya, Takashi ;
Nakayama, Aya ;
Kawamura, Takeshi ;
Sasaki, Kazuki .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 533 (04) :872-878
[33]   A polyamine coating for enhanced capillary electrophoresis-electrospray ionization-mass spectrometry of proteins and peptides [J].
Ullsten, S ;
Zuberovic, A ;
Wetterhall, M ;
Hardenborg, E ;
Markides, KE ;
Bergquist, J .
ELECTROPHORESIS, 2004, 25 (13) :2090-2099
[34]   Evaluation of volatile eluents and electrolytes for high-performance liquid chromatography-electrospray ionization mass spectrometry and capillary electrophoresis-electrospray ionization mass spectrometry of proteins - II. Capillary electrophoresis [J].
Huber, CG ;
Premstaller, A ;
Kleindienst, G .
JOURNAL OF CHROMATOGRAPHY A, 1999, 849 (01) :175-189
[35]   Sheathless capillary electrophoresis-mass spectrometry for anionic metabolic profiling [J].
Gulersonmez, Mehmet Can ;
Lock, Stephen ;
Hankemeier, Thomas ;
Ramautar, Rawi .
ELECTROPHORESIS, 2016, 37 (7-8) :1007-1014
[36]   Analysis of 8-aminonaphthalene-1,3,6-trisulfonate-derivatized oligosaccharides by capillary electrophoresis-electrospray ionization quadrupole ion trap mass spectrometry [J].
Che, FY ;
Song, JF ;
Zeng, R ;
Wang, KY ;
Xia, QC .
JOURNAL OF CHROMATOGRAPHY A, 1999, 858 (02) :229-238
[37]   Live Monitoring of Anionic Living Polymerizations by Electrospray-Ionization Mass Spectrometry [J].
Eisele, Niklas F. F. ;
Peters, Matthias ;
Koszinowski, Konrad .
CHEMISTRY-A EUROPEAN JOURNAL, 2023, 29 (13)
[38]   Determining Initiator Efficiency in Radical Polymerization by Electrospray-Ionization Mass Spectrometry [J].
Buback, Michael ;
Frauendorf, Holm ;
Guenzler, Fabian ;
Huff, Felix ;
Vana, Philipp .
MACROMOLECULAR CHEMISTRY AND PHYSICS, 2009, 210 (19) :1591-1599
[39]   Recent advances in capillary electrophoresis electrospray mass spectrometry [J].
Banks, JF .
ELECTROPHORESIS, 1997, 18 (12-13) :2255-2266
[40]   An electrospray-ionization mass spectrometry analysis of triacylglycerols in developing corn kernels [J].
Harrabi, Saoussem ;
Boukhchina, Sadok ;
Kallel, Habib ;
Mayer, Paul M. .
CANADIAN JOURNAL OF ANALYTICAL SCIENCES AND SPECTROSCOPY, 2008, 53 (01) :22-27
12345