ADVANCES IN THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF PHENYLTHIOCARBAMYL AMINO-ACIDS

Some of the general problems commonly encountered in the analysis of phenylthiocarbamyl amino acids are described. This review includes experiences associated with the preparation and storage of derivatives prior to analysis and those originating from the analytical procedure itself. The issue of the quantification of the phenylthiocarbamyl derivatives of tryptophan and cyst(e)ine, together with all other amino acids from the same hydrolysate, is also discussed. The possible reproducibility of the measurements, as a function of the conditions applied is considered.