TGF-BETA INHIBITS PROLIFERATION OF AND PROMOTES DIFFERENTIATION OF HUMAN PROMONOCYTIC LEUKEMIA-CELLS

Transforming growth factor-beta 1 (TGF-beta-1) has been implicated in a variety of responses associated with wound healing and inflammation. Thus, TGF-beta-1 enhances production of several extracellular matrix proteins both in vitro and in vivo, is chemotactic for monocytes, and alters the functioning of lymphocytes. We have examined the ability of TGF-beta-1 to affect the behavior of human THP-1 promonocytic leukemia cells, a cell line with the capacity to differentiate into macrophage-like cells. TGF-beta-1 reduces the growth rate of these cells, induces morphologic changes, and promotes adherence to culture surfaces. In addition, the adherent cell population expresses high levels of esterase activity, acquires the ability to ingest latex beads, and releases elevated levels of interleukin 1. TGF-beta-1-treated cells also express elevated levels of the beta-2 family of integrins. Taken together, these results suggest that TGF-beta-1 is capable of promoting the maturation of promonocytic cells into macrophages. This outcome has implications at wound sites where TGF-beta-1 and.a myriad of other factors interact with many cell types to facilitate healing.