ADDITION OF SERUM TO ELECTROPORATED CELLS ENHANCES SURVIVAL AND TRANSFECTION EFFICIENCY

被引：22
作者：
BAHNSON, AB [1 ]
BOGGS, SS [1 ]
机构：
[1] UNIV PITTSBURGH,GRAD SCH PUBL HLTH,DEPT HUMAN GENET,PITTSBURGH,PA 15261
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)91210-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Optimal electroporation efficiency of many cell types is associated with poor survival. We show that serum rapidly reseals the membranes of electroporated cells and that timely addition of serum following electroporation can improve cell survival and transfection efficiency. © 1990.
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页码：752 / 757
页数：6
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