GENOTYPING OF POOR METABOLIZERS OF DEBRISOQUINE BY ALLELE-SPECIFIC PCR AMPLIFICATION

被引：495

作者：

HEIM, M ^{[1
]}

MEYER, UA ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,DEPT PHARMACOL,KLINGELBERGSTR 70,CH-4056 BASEL,SWITZERLAND

来源：

LANCET | 1990年 / 336卷 / 8714期

关键词：

D O I：

10.1016/0140-6736(90)92086-W

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A method for genotyping poor metabolisers of debrisoquine is based on specific polymerase chain reaction (PCR) amplification of parts of mutant genes for hepatic cytochrome P450IID6. Analysis by restriction fragment length polymorphism allowed identification of only 25% of poor metabolisers, but when it was combined with allele-specific PCR over 95% of poor metabolisers could be identified. The PCR method also allowed the identification of heterozygous carriers of mutant alleles. © 1990.

引用

页码：529 / 532

页数：4

共 22 条

[1] METABOLIC OXIDATION PHENOTYPES AS MARKERS FOR SUSCEPTIBILITY TO LUNG-CANCER [J].