MOLECULAR KARYOTYPE ALTERATIONS INDUCED BY TRANSFORMATION IN ASPERGILLUS-NIDULANS ARE MITOTICALLY STABLE

被引：14
|
作者：
XUEI, XL [1 ]
SKATRUD, PL [1 ]
机构：
[1] ELI LILLY & CO,INFECT DIS RES,INDIANAPOLIS,IN 46285
来源：
CURRENT GENETICS | 1994年 / 26卷 / 03期
关键词：
ASPERGILLUS NIDULANS; MOLECULAR KARYOTYPE; GENETIC TRANSFORMATION; MITOTIC STABILITY;
D O I：
10.1007/BF00309551
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Clamped homogeneous electric field (CHEF)-gel electrophoresis was used to define the electrophoretic molecular karyotype of Aspergillus nidulans strain OC-1 before and after protoplast-based genetic transformation. The transforming DNA caused alterations in the molecular karyotypes in all transformants examined. Rather dramatic changes were observed in karyotypes, including apparent chromosome loss, massive size alterations, and the appearance of large chromosomes. Changes in molecular karyotype were mitotically stable.
引用
收藏
页码：225 / 227
页数：3
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