RANDOM AMPLIFIED POLYMORPHIC DNA AND GENETIC DIVERSITY IN INDIAN MUSA GERMPLASM

被引：51
作者：
BHAT, KV [1 ]
JARRET, RL [1 ]
机构：
[1] USDA ARS,GRIFFIN,GA 30223
来源：
GENETIC RESOURCES AND CROP EVOLUTION | 1995年 / 42卷 / 02期
关键词：
RANDOM AMPLIFIED POLYMORPHIC DNA; BANANA; GENETIC DIVERSITY; CULTIVAR IDENTIFICATION;
D O I：
10.1007/BF02539514
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Fifty-seven accessions of Musa including cultivated clones of 6 genomic groups (AA, AB, AAA, AAB, ABB, ABBB), M. balbisiana Colla (BB), M. acuminata Colla ssp. banksii F. Muell. (AA), M. acuminata Colla ssp. malaccensis Ridl. (AA) and M. velutina Wendl. & Drude were examined for random amplified polymorphic DNA (RAPD) genetic markers using PCR with sixty 10-mer random primers. Forty-nine of 60 tested primers gave reproducible DNA amplification patterns. The number of bands resolved per amplification was primer dependent and varied from 1 to a maximum of 24. The size range of the amplification products also differed with the selected primer sequence/genotype and ranged from 0.29 to 3.0 kb. RAPD data were used to generate Jaccard's similarity coefficients which were analyzed phenetically. Phenetic analysis separated clones into distinct groupings that were in agreement with clusterings revealed when data were subsequently analyzed by principal coordinate analysis (PCO). In both the phenetic and the PCO analyses, previously unclassified cultivars grouped with cultivars previously classified for their genomic group based on morphological keys. The implications of RAPD analysis for Musa germplasm classification, clonal identification, and management are discussed.
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页码：107 / 118
页数：12
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共 39 条
  • [1] Akkaya M.S., Bhagwat A.A., Cregan P.B., Length polymorphisms of simple sequence repeat DNA in soybean, Genetics, 132, pp. 1131-1139, (1992)
  • [2] Bhat K.V., Bhat S.R., Chandel K.P.S., Survey of isozyme polymorphism for clonal identification in Musa. I. Esterase, acid phosphatase and catalase, J. Hort. Sci., 67, pp. 501-507, (1992)
  • [3] Bhat K.V., Bhat S.R., Chandel K.P.S., Survey of isozyme polymorphism for clonal identification in Musa. II. Peroxidase, superoxide dismurase, shikimate dehydrogenase and malate dehydrogenase, J. Hort. Sci., 67, pp. 737-744, (1992)
  • [4] Bonner J.W., Warner R.M., Brewbaker J.L., A chemosystematic study of Musa cultivars, HortScience, 9, pp. 325-328, (1974)
  • [5] Brunk C.F., Jones K.C., James T.W., Assay for nanogram quantities of DNA in cellular homogenates, Anal. Biochem., 92, pp. 497-500, (1979)
  • [6] Chakravorty A.K., Origin of the cultivated bananas of Southeast Asia, Indian J. Genet., 11, pp. 34-46, (1951)
  • [7] Daniells J.W., The Cavendish subgroup: Distinct and less distinct cultivars, Identification of Genetic Diversity in the Genus Musa, pp. 29-35, (1990)
  • [8] Demeke T., Ada R.P., Chibbar R., Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study in Brassica, Theor. Appl. Genet., 84, pp. 990-994, (1992)
  • [9] Gawel N., Jarret R.L., Cytoplasmic genetic diversity in bananas and plantains, Euphytica, 52, pp. 19-23, (1991)
  • [10] Gawel N., Jarret R.L., Whittemore A., Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa, Theor. Appl. Genet., 84, pp. 286-290, (1992)
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