MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF NUCLEIC-ACIDS WITH WAVELENGTHS IN THE ULTRAVIOLET AND INFRARED

被引：260

作者：

NORDHOFF, E ^{[1
]}

INGENDOH, A ^{[1
]}

CRAMER, R ^{[1
]}

OVERBERG, A ^{[1
]}

STAHL, B ^{[1
]}

KARAS, M ^{[1
]}

HILLENKAMP, F ^{[1
]}

CRAIN, PF ^{[1
]}

机构：

[1] UNIV UTAH,DEPT MED CHEM,SALT LAKE CITY,UT 84112

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 12期

关键词：

D O I：

10.1002/rcm.1290061212

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of different matrices have been tested and compared for ultraviolet and infrared (UV and IR) matrix-assisted laser desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from E. coli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR-MALDI-MS was determined.

引用

页码：771 / 776

页数：6

共 14 条

[1] BORNSEN KO, 1990, CHIMIA, V44, P412
[2] HILLENKAMP F, 1990, BIOLOGICAL MASS SPECTROMETRY /, P49
[3] ENERGETICS OF GRAMICIDIN-S AFTER UV LASER DESORPTION FROM A FERULIC ACID MATRIX
HUTHFEHRE, T
BECKER, CH
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1991, 5 (08) : 378 - 382
[4] HUTHFEHRE T, 1992, DESORPTION 92, P9
[5] LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF LARGE BIOMOLECULES
KARAS, M
BAHR, U
[J]. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 1990, 9 (10) : 321 - 325
[6] LASER DESORPTION IONIZATION OF PROTEINS WITH MOLECULAR MASSES EXCEEDING 10000 DALTONS
KARAS, M
HILLENKAMP, F
[J]. ANALYTICAL CHEMISTRY, 1988, 60 (20) : 2299 - 2301
[7] TIME-OF-FLIGHT MASS-SPECTROMETRY OF NUCLEIC-ACIDS BY LASER ABLATION AND IONIZATION FROM A FROZEN AQUEOUS MATRIX
NELSON, RW
THOMAS, RM
WILLIAMS, P
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1990, 4 (09) : 348 - 351
[8] SPECIFIC PHOTOREACTIONS BETWEEN PSORALENS AND YEAST-TRANSFER RNAPHE
NIELSEN, PE
LEICK, V
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1982, 106 (01) : 179 - 185
[9] OVERBERG A, 1990, RAPID COMMUN MASS SP, V5, P128
[10] MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS-SPECTROMETRY OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES
PARR, GR
FITZGERALD, MC
SMITH, LM
[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (06) : 369 - 372

← 1 2 →