PECTIN METHYLESTERASE IN CALLI AND GERMINATING-SEEDS OF OROBANCHE-AEGYPTIACA

被引:31
作者
BENHOD, G [1 ]
LOSNER, D [1 ]
JOEL, DM [1 ]
MAYER, AM [1 ]
机构
[1] ARO, REG EXPTL STN, DEPT WEED RES, NEWE YAAR, ISRAEL
关键词
OROBANCHE-AEGYPTIACA; OROBANCHACEAE; BROOMRAPE; CALLUS; PECTIN METHYLESTERASE; POLYGALACTURONASE; GERMINATING SEEDS; ENZYME SECRETION;
D O I
10.1016/0031-9422(93)85145-H
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The presence and secretion of pectin methylesterase was compared in infective calli and seed Orobanche aegyptiaca and of O. cumana. Enzyme activity was of the same order of magnitude in both tissues. Calli were able to secrete enzyme into liquid medium, but there was little secretion into solid medium. Pre-incubation of calli in the presence of pectin caused the earlier appearance of enzyme activity in liquid medium, but did not appear to change the amount of enzyme secreted. It is speculated that pectin methylesterase may have a function in the infection of the host by the parasite, allowing it to intrude between host cells.
引用
收藏
页码:1399 / 1402
页数:4
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