EFFECT OF PUPARIA INCUBATION-TEMPERATURE - INCREASED INFECTION-RATES OF TRYPANOSOMA-CONGOLENSE IN GLOSSINA-MORSITANS CENTRALIS, G-FUSCIPES-FUSCIPES AND G-BREVIPALPIS

Puparia of Glossina morsitans centralis (Machado), G.fuscipes fuscipes (Newstead) and G.brevipalpis (Newstead) were incubated at 25 +/- 1-degrees-C, 28 +/- 1:25 +/- 1-degrees-C, day:night or 29 +/- 1-degrees-C throughout the puparial period, and maintained at 70-80% relative humidity. Puparial mortality was higher at 29 than at 25-degrees-C (optimum temperature) in all three species, particularly in G.f.fuscipes and G.brevipalpis. Adults of G.m.centralis from puparia incubated at 29-degrees-C, and those of this subspecies, G.f.fuscipes and G.brevipalpis from puparia incubated at 28:25-degrees-C, day:night or 25-degrees-C throughout, were infected as tenerals (27 h old) by feeding them at the same time on goats infected with Trypanosoma congolense (Broden) IL 1180 after the parasites were detected in the wet blood film. Infection rates on day 25 post-infected feed were higher in G.m.centralis from puparia incubated at 29-degrees-C and in adults of the three different tsetse species from puparia incubated at 28:25-degrees-C, day:night, than in those from puparia incubated at 25-degrees-C. However, in G.f.fuscipes the labral and hypopharyngeal infection rates were not significantly different from those of the tsetse produced by puparia kept at 25-degrees-C.