IDENTIFICATION OF FILARIAL LARVAE IN VECTORS BY DNA HYBRIDIZATION

被引:4
作者
DISSANAYAKE, S
PIESSENS, WF
机构
[1] Senarath Dissonayake and Willy F. Piessens are, the Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115
来源
PARASITOLOGY TODAY | 1992年 / 8卷 / 02期
关键词
D O I
10.1016/0169-4758(92)90092-G
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Infectivity rates of insect vectors are the best criteria by which to assess the transmission of filarial parasites and the efficacy of filariasis control programs. Currently available DNA probes can be used to estimate the proportion of vectors containing larvae of a given filarial species but provide no information on three other important variables in the transmission dynamics of filarial nematodes: the developmental stage, the location and the actual number of larvae that are present in the vector, all of which can be reliably determined by microscopy. However, species identification is often difficult and sometimes impossible by conventional microscopy, which requires morphologically intact specimens. DNA is tough and DNA probing can identify worms that are dead and that have lost morphologic integrity; it also permits multiple analyses of the same specimen with different probes and has the potential for simultaneous processing of very large numbers of samples. Here, Senarath Dissanayake and Willy Piessens outline the route to the development of species- and life cycle stage-specific DNA/RNA probing reagents; and simple, reliable and quantitative technologies that can supplement and ultimately replace microscopic dissection.
引用
收藏
页码:67 / 69
页数:3
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