HIGH-RESOLUTION MAPPING OF THE PHYSICAL LOCATION OF THE TOMATO CF-2 GENE

被引：31
作者：
DIXON, MS
JONES, DA
HATZIXANTHIS, K
GANAL, MW
TANKSLEY, SD
JONES, JDG
机构：
[1] JOHN INNES CTR PLANT SCI RES, SAINSBURY LAB, NORWICH NR4 7UH, NORFOLK, ENGLAND
[2] INST PLANT GENET & CROP PLANT RES, GATERSLEBEN, GERMANY
[3] CORNELL UNIV, DEPT PLANT BREEDING & BIOMETRY, ITHACA, NY 14853 USA
来源：
MOLECULAR PLANT-MICROBE INTERACTIONS | 1995年 / 8卷 / 02期
关键词：
CLADOSPORIUM FULVUM; TOMATO LEAF MOLD;
D O I：
10.1094/MPMI-8-0200
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To isolate the tomato Cf-2 resistance gene by map-based cloning, plants recombinant for RFLP markers close to Cf-2 were selected by exploiting the flanking morphological markers yv (yellow virescent) and tl (thiaminless). Using these recombinants, a high-resolution linkage map of the region encompassing the Cf-2 gene has been generated containing several new RFLP markers. Mapping of two YAC clones carrying Lycopersicon esculentum and L. peruvianum DNA, indicates that in both genotypes the physical distance between the two closest flanking markers is less than 40 kb. This study also positions Cf-2 relative to the Mi gene, which confers resistance to root-knot nematodes.
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收藏
页码：200 / 206
页数：7
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