BASAL DEPHOSPHORYLATION CONTROLS SLOW GATING OF L-TYPE CA2+ CHANNELS IN HUMAN VASCULAR SMOOTH-MUSCLE

被引：12

作者：

GROSCHNER, K ^{[1
]}

SCHUHMANN, K ^{[1
]}

BAUMGARTNER, W ^{[1
]}

PASTUSHENKO, V ^{[1
]}

SCHINDLER, H ^{[1
]}

ROMANIN, C ^{[1
]}

机构：

[1] UNIV LINZ, INST BIOPHYS, A-4040 LINZ, AUSTRIA

来源：

FEBS LETTERS | 1995年 / 373卷 / 01期

关键词：

L-TYPE CA2+ CHANNEL; PROTEIN PHOSPHATASE; CELLULAR REGULATION; VASCULAR SMOOTH MUSCLE; PATCH CLAMP;

D O I：

10.1016/0014-5793(95)01012-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of cellular phosphatase activity in regulation of smooth muscle L-type Ca2+ channels was investigated using tautomycin, a potent and specific inhibitor of serin/threonin phosphatases type 1 and 2A. Tautomycin (1-100 mM) inhibited Ca2+ channel activity in smooth muscle cells isolated from human umbilical vein, Tautomycin-induced inhibition of Ca2+ channel activity was due to a reduction of channel availability which originated mainly from prolongation of the lifetime of unavailable states of the channel. Pretreatment of smooth muscle cells with the protein kinase inhibitor H-7 (10 mu M) prevented the inhibitory effect of tantomycin., Our results suggest modulation of slow gating between available and unavailable states as a mechanism of phosphorylation-dependent down-regulation of Ca2+ channels in vascular smooth muscle.

引用

页码：30 / 34

页数：5

共 16 条

[1] HISTAMINE INDUCES K+, CA2+, AND CL- CURRENTS IN HUMAN VASCULAR ENDOTHELIAL-CELLS - ROLE OF IONIC CURRENTS IN STIMULATION OF NITRIC-OXIDE BIOSYNTHESIS [J].