DETECTION OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS BY THE POLYMERASE CHAIN-REACTION

被引：20

作者：

NADINDAVIS, SA ^{[1
]}

CHANG, SC ^{[1
]}

SMITH, H ^{[1
]}

JACOBS, RM ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT PATHOL,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1993年 / 42卷 / 2-3期

基金：

加拿大自然科学与工程研究理事会;

关键词：

BOVINE; LENTIVIRUS; POLYMERASE CHAIN REACTION;

D O I：

10.1016/0166-0934(93)90043-Q

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This report describes a test for the bovine lentivirus, bovine immunodeficiency-like virus, in which polymerase chain reaction (PCR) technology is employed. Two pairs of oligonucleotide primers directed to sequences within the coding regions of the gag and pol genes generated the expected PCR products from molecular BIV clones and from DNA of BIV-infected cell cultures but not from DNA of uninfected cultures. Data indicating the specificity and sensitivity of these PCRs for BIV detection and the potential utility of this technology for diagnostic applications are presented.

引用

页码：323 / 336

页数：14

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