EFFECTS OF CALCIUM ENTRY BLOCKERS AND CALMODULIN INHIBITORS ON CYTOKININ-ENHANCED ALKALOID ACCUMULATION IN CATHARANTHUS-ROSEUS CELL-CULTURES

被引：0

作者：

MERILLON, JM ^{[1
]}

LIU, D ^{[1
]}

HUGUET, F ^{[1
]}

CHENIEUX, JC ^{[1
]}

RIDEAU, M ^{[1
]}

机构：

[1] FAC MED TOURS,BIOPHYS MED LAB,INSERM,U316,F-37042 TOURS,FRANCE

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 1991年 / 29卷 / 03期

关键词：

INDOLE ALKALOIDS; CALCIUM UPTAKE; PROTOPLASTS;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In two cell lines of Catharanthus roseus, the accumulation of indole alkaloids is enhanced by adding a cytokinin to the culture medium. Cells grown under conditions which restricted the extracellular Ca2+ fail to accumulate alkaloids, this inhibition being overcome through re-addition of calcium. Inorganic ions, La3+ and Co2+, as well as the calcium channel inhibitors, verapamil, pimozide, bepridil and flunarizine inhibit Ca-45(2+) uptake in C. roseus protoplasts. These compounds, together with dihydropyridines, inhibit the effect of cytokinin on alkaloid accumulation, but the dihydropyridines do not block the Ca2+ influx and specific binding sites for the tritiated drug (+) PN 200-110 cannot be identified. Moreover, several calmodulin inhibitors inhibit the alkaloid synthesis. The significance of Ca2+ ions in the cytokinin-enhanced alkaloid accumulation is discussed.

引用

页码：289 / 296

页数：8

共 38 条

[1] RELEASE OF SUCROSE FROM VICIA-FABA L LEAF-DISKS [J].

ANDERSON, JM .

PLANT PHYSIOLOGY, 1983, 71 (02) :333-340

[2] PITFALLS OF THE CA-45 UPTAKE METHOD [J].

BORLE, AB .

CELL CALCIUM, 1981, 2 (02) :187-196

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4]

Davies P. J., 1988, PLANT HORMONES THEIR

[5] CALCIUM AND THE CELL-WALL [J].

DEMARTY, M ;

MORVAN, C ;

THELLIER, M .

PLANT CELL AND ENVIRONMENT, 1984, 7 (06) :441-448

[6]

Elliot DC, 1986, MOL CELLULAR ASPECTS, P285

[7] CYTOKININ AND FUSICOCCIN EFFECTS ON CALCIUM-TRANSPORT IN AMARANTHUS PROTOPLASTS [J].

ELLIOTT, DC ;

YAO, YG .

PLANT SCIENCE, 1989, 65 (02) :243-252

[8] INHIBITION OF CYTOKININ-REGULATED RESPONSES BY CALMODULIN-BINDING COMPOUNDS [J].

ELLIOTT, DC .

PLANT PHYSIOLOGY, 1983, 72 (01) :215-218

[9] DISTINCTION OF 2 COMPONENTS OF PASSIVE CA-2+ TRANSPORT INTO HUMAN-ERYTHROCYTES BY CA-2+ ENTRY BLOCKERS [J].

ENGELMANN, B ;

DUHM, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 981 (01) :36-42

[10] NEUROLEPTICS OF THE DIPHENYLBUTYLPIPERIDINE SERIES ARE POTENT CALCIUM-CHANNEL INHIBITORS [J].

GALIZZI, JP ;

FOSSET, M ;

ROMEY, G ;

LADURON, P ;

LAZDUNSKI, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) :7513-7517

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