PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES TO THE TOXIN COREGULATED PILUS (TCP) OF VIBRIO-CHOLERAE THAT PROTECT AGAINST EXPERIMENTAL CHOLERA IN INFANT MICE

被引：19

作者：

SUN, D ^{[1
]}

TILLMAN, DM ^{[1
]}

MARION, TN ^{[1
]}

TAYLOR, RK ^{[1
]}

机构：

[1] UNIV TENNESSEE,CTR HLTH SCI,HLTH SCI CTR,DEPT MICROBIOL & IMMUNOL,MEMPHIS,TN 38163

来源：

SERODIAGNOSIS AND IMMUNOTHERAPY IN INFECTIOUS DISEASE | 1990年 / 4卷 / 01期

关键词：

monoclonal antibodies; pilus; vaccine; Vibrio cholerae;

D O I：

10.1016/0888-0786(90)90051-O

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Six monoclonal antibodies (MAbs) directed against purified TCP (toxin coregulated pilus) of classical V. cholerae strain 0395 were developed and characterized. All of these MAbs recognized the major pilin subunit (TcpA) by western immunoblot. In the infant mouse experimental cholera model, only two of the MAbs (169·1 and 16·1) were shown to be protective although all six recognized TCP quaternary structure as determined by ELISA or immunoelectron microscopy. The results suggest that potentially protective epitopes can be identified in the TCP structure, specifically within TcpA, and that neutralization of the corresponding functional domains is sufficient to provide protection from cholera. © 1990.

引用

页码：73 / 81

页数：9

共 18 条

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