THE PRODUCTION AND CHARACTERIZATION OF RABBIT ANTISERUM AND MURINE MONOCLONAL-ANTIBODIES TO HAEMOPHILUS-DUCREYI

被引：9

作者：

FINN, GY ^{[1
]}

KARIM, QN ^{[1
]}

EASMON, CSF ^{[1
]}

机构：

[1] ST MARYS HOSP,SCH MED,WRIGHT FLEMING INST,DEPT MED MICROBIOL,PRAED ST,LONDON W2 1PG,ENGLAND

来源：

JOURNAL OF MEDICAL MICROBIOLOGY | 1990年 / 31卷 / 03期

关键词：

D O I：

10.1099/00222615-31-3-219

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Rabbit antiserum and murine monoclonal antibodies were raised against a strain of Haemophilus ducreyi. The antiserum gave high immunofluorescence titres and strong dot blot reactions with all H. ducreyi strains tested and the only cross reaction was with Bordetella pertussis. Three monoclonal antibodies, all of isotype IgG2a, also gave high immunofluorescence titres with H. ducreyi but did not cross react with any other species tested. Immunoblotting showed the monoclonal antibodies to react with a single polypeptide band of mol. wt 29000 in the outer-membrane fraction of H. ducreyi. These antibodies have potential for use as diagnostic reagents and for investigating the pathogenicity of H. ducreyi.

引用

页码：219 / 224

页数：6

共 18 条

[1] SUBTYPING ISOLATES OF HEMOPHILUS-INFLUENZAE TYPE-B BY OUTER-MEMBRANE PROTEIN PROFILES [J].

BARENKAMP, SJ ;

MUNSON, RS ;

GRANOFF, DM .

JOURNAL OF INFECTIOUS DISEASES, 1981, 143 (05) :668-676

[2] HOW RELIABLE IS MORPHOLOGICAL DIAGNOSIS OF PENILE ULCERATIONS [J].

CHAPEL, TA ;

BROWN, WJ ;

JEFFRIES, C ;

STEWART, JA .

SEXUALLY TRANSMITTED DISEASES, 1977, 4 (04) :150-152

[3]

DENYS GA, 1978, HEALTH LAB SCI, V15, P128

[4]

HAMMOND GW, 1978, J CLIN MICROBIOL, V7, P39

[5]

HAMMOND GW, 1980, REV INFECT DIS, V2, P867

[6] MONOCLONAL-ANTIBODIES REACTIVE WITH ALL STRAINS OF HEMOPHILUS-DUCREYI [J].

HANSEN, EJ ;

LOFTUS, TA .

INFECTION AND IMMUNITY, 1984, 44 (01) :196-198

[7] EVALUATION OF A DIRECT IMMUNOFLUORESCENCE TEST FOR DIAGNOSING GONORRHEA [J].

ISON, CA ;

MCLEAN, K ;

GEDNEY, J ;

MUNDAY, PE ;

COGHILL, D ;

SMITH, R ;

HARRIS, JRW ;

EASMON, CSF .

JOURNAL OF CLINICAL PATHOLOGY, 1985, 38 (10) :1142-1145

[8]

ISON CA, 1987, GENITOURIN MED, V63, P196

[9] DERIVATION OF SPECIFIC ANTIBODY-PRODUCING TISSUE-CULTURE AND TUMOR LINES BY CELL-FUSION [J].

KOHLER, G ;

MILSTEIN, C .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1976, 6 (07) :511-519

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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