The complexation of protonated peptides with saccharides in the gas phase decreases the rates of hydrogen/deuterium exchange reactions

被引：23

作者：

Green, MK ^{[1
]}

Penn, SG ^{[1
]}

Lebrilla, CB ^{[1
]}

机构：

[1] UNIV CALIF DAVIS,DEPT CHEM,DAVIS,CA 95616

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1995年 / 6卷 / 12期

关键词：

D O I：

10.1016/1044-0305(95)00632-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gas-phase noncovalently bound complexes are probed by hydrogen/deuterium exchange. The complexes, composed of a protonated amino acid and a monosaccharide, are investigated to observe the effects of complexation on the rates of exchange. Rate constants are determined and compared for complexed and uncomplexed amino acids. The overall rate constant, which corresponds to exchange of a specific number of hydrogens, is deconvoluted to yield site-specific rate constants. Complexation of amino acids with saccharides significantly decreases the rate constants of the exchange. Results of molecular orbital calculations are provided to explain the decrease in the rates.

引用

页码：1247 / 1251

页数：5

共 19 条

[1] MASS-SPECTROMETRIC CHARACTERIZATION OF A PROTEIN LIGAND INTERACTION
ANDEREGG, RJ
WAGNER, DS
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (04) : 1374 - 1377
[2] STRUCTURAL AND ENERGETIC CONSTRAINTS ON GAS-PHASE HYDROGEN-DEUTERIUM EXCHANGE-REACTIONS OF PROTONATED PEPTIDES WITH D2O, CD3OD, CD3CO2D, AND ND3
CAMPBELL, S
RODGERS, MT
MARZLUFF, EM
BEAUCHAMP, JL
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (21) : 9765 - 9766
[3] HYDROGEN-DEUTERIUM EXCHANGE OF MASS-SELECTED PEPTIDE IONS WITH ND3 IN A TANDEM SECTOR MASS-SPECTROMETER
CHENG, XH
FENSELAU, C
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY AND ION PROCESSES, 1992, 122 : 109 - 119
[4] EVALUATION OF AM1 CALCULATED PROTON AFFINITIES AND DEPROTONATION ENTHALPIES
DEWAR, MJS
DIETER, KM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (25) : 8075 - 8086
[5] THE DEVELOPMENT AND USE OF QUANTUM-MECHANICAL MOLECULAR-MODELS .76. AM1 - A NEW GENERAL-PURPOSE QUANTUM-MECHANICAL MOLECULAR-MODEL
DEWAR, MJS
ZOEBISCH, EG
HEALY, EF
STEWART, JJP
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1985, 107 (13) : 3902 - 3909
[6] DETECTION OF NONCOVALENT RECEPTOR LIGAND COMPLEXES BY MASS-SPECTROMETRY
GANEM, B
LI, YT
HENION, JD
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (16) : 6294 - 6296
[7] MASS-SPECTROMETRIC DETECTION OF THE NONCOVALENT GDP-BOUND CONFORMATIONAL STATE OF THE HUMAN H-RAS PROTEIN
GANGULY, AK
PRAMANIK, BN
TSARBOPOULOS, A
COVEY, TR
HUANG, E
FUHRMAN, SA
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (16) : 6559 - 6560
[8] SITE-SPECIFICITY IN THE H-D EXCHANGE-REACTIONS OF GAS-PHASE PROTONATED AMINO-ACIDS WITH CH3OD
GARD, E
WILLARD, D
BREGAR, J
GREEN, MK
LEBRILLA, CB
[J]. ORGANIC MASS SPECTROMETRY, 1993, 28 (12): : 1632 - 1639
[9] GAS-PHASE HYDROGEN-DEUTERIUM EXCHANGE AS A MOLECULAR PROBE FOR THE INTERACTION OF METHANOL AND PROTONATED PEPTIDES
GARD, E
GREEN, MK
BREGAR, J
LEBRILLA, CB
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (07) : 623 - 631
[10] OBSERVATION OF THE HEME GLOBIN COMPLEX IN NATIVE MYOGLOBIN BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY
KATTA, V
CHAIT, BT
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (22) : 8534 - 8535

← 1 2 →