PHOSPHATIDYLSERINE HEADGROUP DIASTEREOMERS TRANSLOCATE EQUIVALENTLY ACROSS HUMAN ERYTHROCYTE-MEMBRANES

被引：11

作者：

HALL, MP ^{[1
]}

HUESTIS, WH ^{[1
]}

机构：

[1] STANFORD UNIV,DEPT CHEM,STANFORD,CA 94305

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1190卷 / 02期

关键词：

PHOSPHATIDYLSERINE STEREOCHEMISTRY; AMINOPHOSPHOLIPID TRANSLOCATOR; ERYTHROCYTE MORPHOLOGY;

D O I：

10.1016/0005-2736(94)90080-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The natural chiral phospholipid substrates for the plasma membrane aminophospholipid translocator are L-alpha-phosphatidyl-L-serine serine and L-alpha-phosphatidylethanolamine. The glyceric D-stereoisomers of these lipids, D-alpha-phosphatidyl-L-serine and D-alpha-phosphatidylethanolamine, are not translocated (Martin, O.C. and Pagano, R.E. (1987) J. Biol. Chem. 262, 5890-5898). We have synthesized a diastereomer of phosphatidylserine, L-alpha-phosphatidyl-D-serine, to study the effects of headgroup stereochemistry on translocation. The diastereomer was synthesized as the dilauroyl (12:0) species, and the translocation was monitored by human erythrocyte morphology changes at 25-degrees-C and 37-degrees-C. Unlike other phosphatidylserine stereoisomers, L-alpha-phosphatidyl-D-serine is translocated to the same degree as the natural L,L-isomer. Incorporation of apparently equal amounts of the L,D- and L,L-diastereomers does produce minor quantitative differences in the cell morphological response, possibly as a result of differences in lipid packing of the two isomers.

引用

页码：243 / 247

页数：5

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