A DROSOPHILA HOMOLOG OF THE YEAST ORIGIN RECOGNITION COMPLEX

被引：123
作者：
GOSSEN, M
PAK, DTS
HANSEN, SK
ACHARYA, JK
BOTCHAN, MR
机构：
[1] UNIV CALIF BERKELEY, DEPT MOLEC & CELL BIOL, DIV BIOCHEM & MOLEC BIOL, BERKELEY, CA 94720 USA
[2] UNIV CALIF BERKELEY, HOWARD HUGHES MED INST, DEPT MOLEC & CELL BIOL, BERKELEY, CA 94720 USA
[3] UNIV CALIF SAN DIEGO, HOWARD HUGHES MED INST, DEPT BIOL, LA JOLLA, CA 92093 USA
[4] UNIV CALIF SAN DIEGO, DEPT NEUROSCI, LA JOLLA, CA 92093 USA
来源：
SCIENCE | 1995年 / 270卷 / 5242期
关键词：
D O I：
10.1126/science.270.5242.1674
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Genes from Drosophila melanogaster have been identified that encode proteins homologous to Orc2p and Orc5p of the Saccharomyces cerevisiae origin recognition complex (ORC). The abundance of the Drosophila Orc2p homolog DmORC2 is developmentally regulated and is greatest during the earliest stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts revealed that DmORC2 is found in a tightly associated complex with five additional polypeptides, much like the yeast ORC. These studies will enable direct testing of the initiator-based model of replication in a metazoan.
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页码：1674 / 1677
页数：4
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