DETERMINATION OF THE VITRONECTIN BINDING-SITE ON PLASMINOGEN-ACTIVATOR INHIBITOR-1 (PAI-1)

被引：48
|
作者：
VANMEIJER, M
GEBBINK, RK
PREISSNER, KT
PANNEKOEK, H
机构：
[1] UNIV AMSTERDAM,ACAD MED CTR,DEPT BIOCHEM K1159,1105 AZ AMSTERDAM,NETHERLANDS
[2] MAX PLANCK GESELL,KERCKHOFF KLIN,HAEMOSTASIS RES UNIT,W-6350 BAD NAUHEIM,GERMANY
来源：
FEBS LETTERS | 1994年 / 352卷 / 03期
关键词：
FIBRINOLYSIS; PAI-1; VITRONECTIN; THROMBIN;
D O I：
10.1016/0014-5793(94)00990-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays, we demonstrate that the anti-PAI-1 MoAbs CLB-5, CLB-10, CLB-2C8 and 11, directed against different epitopes in the region between amino acids 110 and 145, prevent the interaction of PAI-1 with vitronectin. We conclude that the region between amino acids 110 and 145 of PAI-1 harbours an important determinant for the interaction with vitronectin.
引用
收藏
页码：342 / 346
页数：5
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