HIGH-EFFICIENCY TRANSFORMATION OF KLUYVEROMYCES-MARXIANUS BY A REPLICATIVE PLASMID

被引：21

作者：

IBORRA, F

机构：

[1] Laboratoire de Biologie et Génetique Moléculaire, Institut de Génétique et Microbiologie Bat 400, Université Paris 11, Orsay Cedex

来源：

CURRENT GENETICS | 1993年 / 24卷 / 1-2期

关键词：

KLUYVEROMYCES-MARXIANUS; TRANSFORMATION; REPLICATIVE VECTOR;

D O I：

10.1007/BF00324685

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Kluyveromyces marxianus can be transformed with an efficiency of 10(5) transformants/mug of DNA by a replicative plasmid using electroporation. In order to obtain this efficiency, we isolated ura- mutants cells which can be complemented by the URA3 gene from Saccharomyces cerevisiae. The URA3 gene and KARS2, a replicative origin from Kluyveromyces lactis which functions in K. marxianus, were ligated together in a plasmid which can be used as a vector to transform this strain.

引用

页码：181 / 183

页数：3

共 11 条

[1]

BECKER DM, 1991, METHOD ENZYMOL, V194, P182

[2] COORDINATION OF SYNTHESIS OF ENZYMES IN PYRIMIDINE PATHWAY OF E COLI [J].

BECKWITH, JR ;

PARDEE, AB ;

JACOB, F ;

AUSTRIAN, R .

JOURNAL OF MOLECULAR BIOLOGY, 1962, 5 (06) :618-&

[3] A POSITIVE SELECTION FOR MUTANTS LACKING OROTIDINE-5'-PHOSPHATE DECARBOXYLASE ACTIVITY IN YEAST - 5-FLUORO-OROTIC ACID RESISTANCE [J].

BOEKE, JD ;

LACROUTE, F ;

FINK, GR .

MOLECULAR & GENERAL GENETICS, 1984, 197 (02) :345-346

[4] TRANSFORMATION OF KLUYVEROMYCES-FRAGILIS [J].

DAS, S ;

KELLERMANN, E ;

HOLLENBERG, CP .

JOURNAL OF BACTERIOLOGY, 1984, 158 (03) :1165-1167

[5] HIGH-FREQUENCY OF YEAST TRANSFORMATION BY PLASMIDS CARRYING PART OR ENTIRE 2-MU-M YEAST PLASMID [J].

GERBAUD, C ;

FOURNIER, P ;

BLANC, H ;

AIGLE, M ;

HESLOT, H ;

GUERINEAU, M .

GENE, 1979, 5 (03) :233-253

[6] TRANSFORMATION OF INTACT YEAST-CELLS TREATED WITH ALKALI CATIONS [J].

ITO, H ;

FUKUDA, Y ;

MURATA, K ;

KIMURA, A .

JOURNAL OF BACTERIOLOGY, 1983, 153 (01) :163-168

[7] REGULATION OF PYRIMIDINE BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE [J].

LACROUTE, F .

JOURNAL OF BACTERIOLOGY, 1968, 95 (03) :824-&

[8] HIGH-EFFICIENCY TRANSFORMATION OF INTACT YEAST-CELLS BY ELECTRIC-FIELD PULSES [J].

MEILHOC, E ;

MASSON, JM ;

TEISSIE, J .

BIO-TECHNOLOGY, 1990, 8 (03) :223-227

[9] HIGH-EFFICIENCY TRANSFORMATION OF SCHIZOSACCHAROMYCES-POMBE BY ELECTROPORATION [J].

PRENTICE, HL .

NUCLEIC ACIDS RESEARCH, 1992, 20 (03) :621-621

[10] CLONING AND EXPRESSION OF THE STRUCTURAL GENE FOR BETA-GLUCOSIDASE OF KLUYVEROMYCES-FRAGILIS IN ESCHERICHIA-COLI AND SACCHAROMYCES-CEREVISIAE [J].

RAYNAL, A ;

GUERINEAU, M .

MOLECULAR & GENERAL GENETICS, 1984, 195 (1-2) :108-115

← 1 2 →