INHIBITION-KINETICS OF BRAIN BUTYRYLCHOLINESTERASE BY CD2+ AND ZN2+, CA2+ OR MG2+ REACTIVATES THE INHIBITED ENZYME

被引：21
作者：
COKUGRAS, AN
TEZCAN, EF
机构：
[1] Department of Biochemistry, Faculty of Medicine, Hacettepe University, Ankara
来源：
INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1993年 / 25卷 / 08期
关键词：
D O I：
10.1016/0020-711X(93)90589-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. The inhibition kinetics of sheep brain butyrylcholinesterase (BChE) (acylcholine acylhydrolase, EC 3.1.1.8) by Cd2+ and Zn2+ has been studied. 2. K(S) has been determined as 0.14 mM. Cd2+ and Zn2+ were the hyperbolic mixed-type inhibitors of BChE. Ca2+ and Mg2+ had no effect on the enzyme activity in the experimental conditions. 3. But when the enzyme was inhibited by 0.1 mM Cd2+ or Zn2+, Ca2+ and Mg2+ reactivated the inhibited form of BChE.
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页码：1115 / 1120
页数：6
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