ENDOTHELIUM-DEPENDENT RELAXATION RESISTANT TO N-G-NITRO-L-ARGININE IN RAT AORTA

Experiments were designed to determine whether cyclic GMP-independent relaxation is involved in the endothelium-dependent vascular relaxation response of rat aortic strip to acetylcholine. The relaxation response to acetylcholine in the presence of 3 x 10(-4) M N-G-nitro-L-arginine was apparent when the precontraction was induced by norepinephrine at 5 X 10(-9) M or 10(-8) M. The relaxation response to acetylcholine resistant to N-G-nitro-L-arginine was abolished by 10(-6) M atropine, 10 mM tetraethylammonium, or endothelium removal, but was not inhibited by 10(-5) M indomethacin, 3 x 10(-6) M oxyhemoglobin or 10(-5) M glibenclamide. The response was virtually abolished when the vascular strips had been preconstricted with 20 mM KCl. The increase in vascular cyclic GMP levels induced by 10(-5) M acetylcholine was completely abolished by 3 x 10(-4) M N-G-nitro-L-arginine. These results suggest that acetylcholine-induced endothelium-dependent relaxation resistant to N-G-nitro-L-arginine in rat aorta is unmasked when the precontractile force is caused by lower concentrations of norepinephrine and the relaxation is mediated by a cyclic GMP-independent mechanism, possibly an endothelium-derived hyperpolarizing factor.