THE EXPRESSION OF MATRIX METALLOPROTEINASES AND THEIR INHIBITORS BY PIG SYNOVIAL-CELLS AND THEIR REGULATION BY COMBINATIONS OF CYTOKINES AND GROWTH-FACTORS

被引：3
作者：
LEGENDRE, P
RICHARDS, CD
RAFFERTY, JA
DEW, GW
REYNOLDS, JJ
机构：
[1] STRANGEWAYS RES LAB,DEPT CELL & MOLEC BIOL,CAMBRIDGE CB1 4RN,ENGLAND
[2] MCMASTER UNIV,MED CTR,DEPT PATHOL,HAMILTON L8N 3Z5,ONTARIO,CANADA
[3] CHRISTIE HOSP & HOLT RADIUM INST,DEPT CARCINOGENESIS,MANCHESTER M20 9BX,LANCS,ENGLAND
来源：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1993年 / 106卷 / 03期
基金：
英国医学研究理事会;
关键词：
D O I：
10.1016/0305-0491(93)90151-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
1. Pig synovial fibroblasts in culture were studied to determine if they were an easily reproducible model system for studying the actions of cytokines and growth factors on human synovial cells. The biochemical analyses were conducted by activity assays, enzymography and Northern blot. 2. Human recombinant interleukin-1alpha, basic fibroblast growth factor and transforming growth factor-beta1 were studied in combinations because of their known involvement in controlling tissue remodelling. 3. The response of pig fibroblasts to these agents, in terms of the expression of matrix metalloproteinases (collagenase, gelatinase and stromelysin) and their inhibitors (TIMPs), show that they behave similarly enough to human cells for use when supplies of human primary cells are unavailable.
引用
收藏
页码：691 / 704
页数：14
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