AN ACUTELY LETHAL SIMIAN IMMUNODEFICIENCY VIRUS STIMULATES EXPANSION OF V(BETA)7-EXPRESSING AND V(BETA)14-EXPRESSING T-LYMPHOCYTES

被引：19

作者：

CHEN, ZW ^{[1
]}

KOU, ZC ^{[1
]}

SHEN, L ^{[1
]}

REGAN, JD ^{[1
]}

LORD, CI ^{[1
]}

HALLORAN, M ^{[1
]}

LEEPARRITZ, D ^{[1
]}

FULTZ, PN ^{[1
]}

LETVIN, NL ^{[1
]}

机构：

[1] UNIV ALABAMA,DEPT MICROBIOL,BIRMINGHAM,AL 35294

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 16期

关键词：

SUPERANTIGEN; T-CELL RECEPTOR V-BETA REPERTOIRE; AIDS; SIVSMMPBJ14;

D O I：

10.1073/pnas.91.16.7501

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

SIVsmmPBj14, a variant simian immunodeficiency virus isolated from a pig-tailed macaque, stimulates the proliferation of macaque T lymphocytes in vitro and induces an acutely lethal disease in macaques characterized, in part, by lymphadenopathy and splenomegaly. To determine whether SIVsmmPBj14 exhibits superantigen-like activity, in vitro and in vivo studies of T-cell receptor V-beta repertoire were undertaken using PCR-based quantitative methods. Whereas in vitro phytohemagglutinin stimulation of macaque peripheral blood lymphocytes did not cause a perturbation of T-cell receptor V-beta repertoire, SIVsmmPBj14 stimulated the expansion of both CD4(+) and CD8(+) T-lymphocyte subpopulations expressing the V(beta)7 and V(beta)14 gene families. Such V(beta)7 and V(beta)14 expansions could be confirmed by a multiple RNase protection assay. Furthermore, the expansion of the same lymphocyte subpopulations was also detected in peripheral blood lymphocytes and lymph node cells of virus-infected macaques. These observations suggest that SIVsmmPBj14-mediated V-beta expansion may contribute to the induction of an acutely lethal disease in macaques,

引用

页码：7501 / 7505

页数：5

共 24 条

[1] LACK OF SELECTIVE V-BETA DELETION IN PERIPHERAL CD4+ T-CELLS OF HUMAN IMMUNODEFICIENCY VIRUS-INFECTED INFANTS [J].