MATRIX-ASSISTED LASER DESORPTION IONIZATION WITH A MAGNETIC MASS-SPECTROMETER

被引：39

作者：

HILL, JA ^{[1
]}

ANNAN, RS ^{[1
]}

BIEMANN, K ^{[1
]}

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1991年 / 5卷 / 09期

关键词：

D O I：

10.1002/rcm.1290050905

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption ionization has been carried out with a high mass double-focusing magnetic mass spectrometer. The pulsed ion signal, generated by irradiation of the sample (substance P, ubiquitin, and cytochrome c) embedded in 2,5-dihydroxybenzoic acid with a XeF excimer laser (353 nm, 12 ns pulse, 10-160 Hz), was recorded with an integrating array detector. Good resolution of 2600 (full width at half maximum) and high sensitivity (a few pmol) were obtained. The loss of small neutral fragments was observed, supporting the notion that the peak broadening observed in the time-of-flight mass spectrometers commonly used for this ionization mode is due to such metastable decomposition.

引用

页码：395 / 399

页数：5

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