POTENT INHIBITION OF SCRAPIE-ASSOCIATED PRP ACCUMULATION BY CONGO RED

被引:213
作者
CAUGHEY, B
RACE, RE
机构
[1] Rocky Mountain Laboratories, Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana
关键词
CONGO RED; AMYLOID; PRP; SCRAPIE-PRION; THERAPY;
D O I
10.1111/j.1471-4159.1992.tb09437.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transmissible spongiform encephalopathies (prion diseases), Alzheimer's disease, and other amyloidoses result in the accumulation of certain abnormally stable Proteins that are thought by many to play central roles in disease pathogenesis. Using scrapie-infected neuroblastoma cells as a model system, we found that Congo red, an amyloid-binding dye, potently inhibits the accumulation of the scrapie-associated, protease-resistant isoform of protein PrP without affecting the metabolism of the normal isoform. Growth of the cells with submicromolar concentrations of Congo red for 5 days reduced the amount of protease-resistant PrP detected in the cultures by >90%. This activity of Congo red suggests that it selectively disrupts the conversion of PrP to the protease-resistant isoform or destabilizes this isoform once it is made. Potential therapeutic applications of Congo red are discussed.
引用
收藏
页码:768 / 771
页数:4
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