ADAPTATIONS OF GOLDNER MASSON TRICHROME STAIN FOR THE STUDY OF UNDECALCIFIED PLASTIC EMBEDDED BONE

被引：120

作者：

GRUBER, HE

机构：

[1] CEDARS SINAI MED CTR,DEPT PEDIAT,LOS ANGELES,CA 90048

[2] UNIV CALIF LOS ANGELES,DEPT PEDIAT,LOS ANGELES,CA 90024

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1992年 / 67卷 / 01期

关键词：

BONE HISTOLOGY; GOLDNER STAIN; MASSON-GOLDNER TRICHROME STAIN; OSTEOID;

D O I：

10.3109/10520299209110002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Specialized adaptations for application of Goldner's Masson trichrome stain to plastic embedded undecalcified bone specimens are presented. This stain can be used successfully on methyl-glycol methacrylate, glycol methacrylate and Spurr embedded bones. The stain affords the advantage of good cellular staining due to the hematoxylin component with concomitant sharp discrimination of mature bone matrix which stains green, immature new bone matrix which stains red, and calcified cartilage which stains very pale green. Use of red filters during photomicrography aids in bone-osteoid discrimination in black and white photographs.

引用

页码：30 / 34

页数：5

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