IDENTIFICATION OF A CONSERVED EPITOPE IN CLASS-I ALCOHOL-DEHYDROGENASE ISOENZYMES USING MONOCLONAL-ANTIBODIES

被引：0

作者：

ADINOLFI, A

LEONE, N

SWALLOW, D

HOPKINSON, DA

ZAPPONI, MC

FERRI, G

CAMARDELLA, L

RONCHI, S

机构：

[1] CNR, INST BIOCHEM PROT & ENZYMOL, NAPLES, ITALY

[2] FAC VET MED MILAN, INST VET PHYSIOL & BIOCHEM, MILAN, ITALY

[3] UNIV LONDON UNIV COLL, MRC, HUMAN BIOCHEM GENET UNIT, GALTON LAB, LONDON WC1E 6BT, ENGLAND

[4] UNIV PAVIA, DEPT BIOL CHEM, I-27100 PAVIA, ITALY

来源：

EXPERIMENTAL AND CLINICAL IMMUNOGENETICS | 1991年 / 8卷 / 02期

基金：

英国惠康基金;

关键词：

ALCOHOL DEHYDROGENASE; MONOCLONAL ANTIBODIES; EPITOPES; IMMUNOLOGICAL CROSS-REACTIVITY;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two monoclonal antibodies raised against native horse alcohol dehydrogenase (HADH) bind preferentially to the enzyme attached to solid supports and recognize the denatured and carboxymethylated HADH subunits. Both antibodies cross-react with the human class I isoenzymes but do not recognize the class III ADH isoenzyme. Protease digestion, electrophoresis and HPLC have been used to identify the linear epitope which is contained in the sequence Pro344-Glu357 of the HADH subunit.

引用

页码：96 / 106

页数：11

共 38 条

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