IDENTIFICATION OF A CONSERVED EPITOPE IN CLASS-I ALCOHOL-DEHYDROGENASE ISOENZYMES USING MONOCLONAL-ANTIBODIES

被引：0
|
作者：
ADINOLFI, A
LEONE, N
SWALLOW, D
HOPKINSON, DA
ZAPPONI, MC
FERRI, G
CAMARDELLA, L
RONCHI, S
机构：
[1] CNR, INST BIOCHEM PROT & ENZYMOL, NAPLES, ITALY
[2] FAC VET MED MILAN, INST VET PHYSIOL & BIOCHEM, MILAN, ITALY
[3] UNIV LONDON UNIV COLL, MRC, HUMAN BIOCHEM GENET UNIT, GALTON LAB, LONDON WC1E 6BT, ENGLAND
[4] UNIV PAVIA, DEPT BIOL CHEM, I-27100 PAVIA, ITALY
来源：
EXPERIMENTAL AND CLINICAL IMMUNOGENETICS | 1991年 / 8卷 / 02期
基金：
英国惠康基金;
关键词：
ALCOHOL DEHYDROGENASE; MONOCLONAL ANTIBODIES; EPITOPES; IMMUNOLOGICAL CROSS-REACTIVITY;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two monoclonal antibodies raised against native horse alcohol dehydrogenase (HADH) bind preferentially to the enzyme attached to solid supports and recognize the denatured and carboxymethylated HADH subunits. Both antibodies cross-react with the human class I isoenzymes but do not recognize the class III ADH isoenzyme. Protease digestion, electrophoresis and HPLC have been used to identify the linear epitope which is contained in the sequence Pro344-Glu357 of the HADH subunit.
引用
收藏
页码：96 / 106
页数：11
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