HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF BILIRUBIN DISTRIBUTION ON SERUM-PROTEINS

被引：5

作者：

SOLTES, L

SEBILLE, B

FUGEDI, A

机构：

[1] CNRS, PHYSICOCHIM BIOPOLYMERES LAB, F-94320 THIAIS, FRANCE

[2] SLOVAK ACAD SCI, CTR COMP SCI, CS-80936 BRATISLAVA, CZECHOSLOVAKIA

来源：

CLINICA CHIMICA ACTA | 1991年 / 201卷 / 1-2期

关键词：

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); BILIRUBIN DISTRIBUTION; ALBUMIN; BETA-LIPOPROTEIN;

D O I：

10.1016/0009-8981(91)90022-5

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We describe a high-performance liquid chromatographic method for the determination of bilirubin distribution on serum proteins. The procedure is based on both size-exclusion and adsorptive properties of the column packing material LiChrosorb Diol. The elution of all sample constituents is accomplished by means of an aqueous mobile phase containing phosphate buffered solution of adult serum, supplemented by a small amount of bilirubin. The different distribution of bilirubin on albumin, beta-lipoprotein, and as free (loosely bound) pigment fraction is exemplified by assaying normal adult and infant sera.

引用

页码：35 / 44

页数：10

共 10 条

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