Extending the In Vivo Residence Time of Macrophage Membrane-Coated Nanoparticles through Genetic Modification

被引：0

作者：

Duan, Yaou ^{[1
,2
]}

Zhou, Jiarong ^{[1
,2
]}

Zhou, Zhidong ^{[1
,2
]}

Zhang, Edward ^{[1
,2
]}

Yu, Yiyan ^{[1
,2
]}

Krishnan, Nishta ^{[1
,2
]}

Silva-Ayala, Daniela ^{[3
,4
]}

Fang, Ronnie H. ^{[1
,2
]}

Griffiths, Anthony ^{[3
,4
]}

Gao, Weiwei ^{[1
,2
]}

Zhang, Liangfang ^{[1
,2
]}

机构：

[1] Univ Calif San Diego, Dept NanoEngn, Chem Engn Program, San Diego, CA 92093 USA

[2] Univ Calif San Diego, Moores Canc Ctr, San Diego, CA 92093 USA

[3] Boston Univ, Sch Med, Dept Microbiol, Boston, MA 02118 USA

[4] Boston Univ, Sch Med, Natl Emerging Infect Dis Labs, Boston, MA 02118 USA

来源：

SMALL | 2023年

关键词：

cell membrane coating; genetic modification; macrophage; nanoparticles; pharmacokinetics;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nanoparticles coated with natural cell membranes have emerged as a promising class of biomimetic nanomedicine with significant clinical potential. Among them, macrophage membrane-coated nanoparticles hold particular appeal due to their versatility in drug delivery and biological neutralization applications. This study employs a genetic engineering approach to enhance their in vivo residence times, aiming to further improve their performance. Specifically, macrophages are engineered to express proline-alanine-serine (PAS) peptide chains, which provide additional protection against opsonization and phagocytosis. The resulting modified nanoparticles demonstrate prolonged residence times when administered intravenously or introduced intratracheally, surpassing those coated with the wild-type membrane. The longer residence times also contribute to enhanced nanoparticle efficacy in inhibiting inflammatory cytokines in mouse models of lipopolysaccharide-induced lung injury and sublethal endotoxemia, respectively. This study underscores the effectiveness of genetic modification in extending the in vivo residence times of macrophage membrane-coated nanoparticles. This approach can be readily extended to modify other cell membrane-coated nanoparticles toward more favorable biomedical applications.

引用

页数：10