STUDIES ON PLATELET RADIOACTIVE ANTI-IMMUNOGLOBULIN TEST

被引：50

作者：

MUELLERECKHARDT, C

SCHULZ, G

SAUER, KH

DIENST, C

MAHN, I

机构：

[1] Department of Clinical Immunology and Blood Transfusion, University of Giessen, Giessen

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1978年 / 19卷 / 01期

关键词：

D O I：

10.1016/0022-1759(78)90002-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A platelet radioactive antiimmunoglobulin test (PRAT) has recently been introduced. In the present report, a detailed analysis of the influence of varying test conditions (i.e. effeciency of iodination of anti-IgG, cell number, degree of sensitization of platelets, times and temperatures of incubation, frequency of washings) on the results of this technique is presented. A standard procedure based on these findings is outlined. It is shown that the PRAT is two to four times more sensitive than platelet complement fixation for the detection of HLA antibodies. © 1978.

引用

页码：1 / 11

页数：11

共 11 条

[1]

COLOMBAN.J, 1971, TRANSPLANT P, V3, P121

[2]

MAHN I, 1975, HAEMOSTASIS, V4, P40

[3] IN VIVO BEHAVIOR OF I131-FIBRINOGEN [J].