SUITABILITY OF DIFFERENT PROTEIN A-GOLD MARKERS FOR IMMUNOGOLD-SILVER STAINING IN PARAFFIN SECTIONS

被引：1
作者：
BRETER, H
ERDMANN, B
机构：
[1] Department of Electron Microscopy, Max Delhrück Center for Molecular Medicine, D-1115, Berlin-Buch
来源：
BIOTECHNIC & HISTOCHEMISTRY | 1993年 / 68卷 / 04期
关键词：
IMMUNOHISTOCHEMISTRY; PROTEIN A-GOLD; SILVER ENHANCEMENT; PARAFFIN SECTIONS;
D O I：
10.3109/10520299309104699
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An incubation protocol to immunolabel Lowicryl semithin sections was applied to paraffin probes. To improve the labeling density, colloidal gold complexes of different preparations and sizes were compared. The type of colloidal gold preparation used was found to affect the specificity of the immunostaining. Gold colloid of 5 nm diameter particle size prepared with white phosphorus minimized nonspecific background labeling of beta-casein in paraffin embedded sections of the mammary epithelium of pregnant mice. Gold colloids of 5 nm and 9 nm diameter particle size prepared in varying concentrations of tannic acid generated significant nonspecific staining in similar tissue preparations.
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页码：206 / 210
页数：5
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